doi: 10.1016/1046-5928(90)90043-x.
One-step fractionation method for isolating H1 histones from chromatin under nondenaturing conditions
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- PMID: 2152183
- DOI: 10.1016/1046-5928(90)90043-x
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One-step fractionation method for isolating H1 histones from chromatin under nondenaturing conditions
Protein Expr Purif.
1990 Sep.
Abstract
By combining conventional methods of chromatin preparation with ion-exchange chromatography in CM-Sephadex C-25, we have been able to isolate and fractionate histone H1 variants from different sources. This method of fractionation is very simple and allows one to obtain, very rapidly, large amounts of these histones in a native nondenatured conformation.
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