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. 2011 May;12(5):393-8.
doi: 10.1631/jzus.B1000179.

Molecular authentication of geo-authentic Scrophularia ningpoensis

Chuan Chen  1 , Li-na Duan, Xiao-long Zhou, Bing-long Chen, Cheng-xin Fu
Affiliations

Molecular authentication of geo-authentic Scrophularia ningpoensis

Chuan Chen et al. J Zhejiang Univ Sci B. 2011 May.

Abstract

Scrophularia ningpoensis has long been used in the Chinese Materia Medica for inflammation. Like other herbal medicines, S. ningpoensis collected from different localities may considerably differ in their therapeutic efficacy, and the one grown in Zhejiang Province is recognized as geo-authentic. However, it is difficult to confirm the geographical authenticity by similar morphological characteristics. In the present study, inter-simple sequence repeat (ISSR) markers were conducted to detect S. ningpoensis from different origins. A 1 259-bp fragment amplified by primer UBC874 was found only in geo-authentic ones. By cloning and sequencing that specific band, sequence characterized amplified region (SCAR) markers were designed to distinguish geo-authentic S. ningpoensis from others. This is a rapid and easy method that can be used to identify the geographical authenticity of S. ningpoensis.

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Figures

Fig. 1
Fig. 1
PCR profiles of UBC874 in S. ningpoensis Arrow indicates the specific band, which is only in samples from Zhejiang Province. M: DNA marker. YC, RC, GM, XJ, PA, HB, and SX are locality codes as shown in Table 1
Fig. 2
Fig. 2
Banding pattern of S. ningpoensis originated from Zhejiang Province (showing a distinct and reproducible band) and other provinces (showing no positive marker) with the designed primers CC874u/CC874d M: DNA marker. YC, RC, GM, PA, XJ, HB, SX, JF, JX, HN, AH, TM, DP, and MT are locality codes as shown in Table 1
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