Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2011 May 15;124(Pt 10):1607-11.
doi: 10.1242/jcs.080085.

Super-resolution microscopy at a glance

Catherine G Galbraith  1 , James A Galbraith
Affiliations
Review

Super-resolution microscopy at a glance

Catherine G Galbraith et al. J Cell Sci. .
No abstract available

PubMed Disclaimer

Figures

Fig. 1.
Fig. 1.
Pathways to consider when choosing a super-resolution method. Super-resolution microscopy either visualizes ensembles or individual molecules. Within these categories the different technologies require different fluorescent probes. In general there is an inverse relationship between image acquisition speed and image resolution. The key to obtaining a super-resolution image is to understand the trade-offs between probes, speed and resolution. Each of the methods shown is currently available from one or more commercial vendors, and more companies will undoubtedly enter the field.
None
See this image and copyright information in PMC

References

    1. Bates M., Huang B., Dempsey G. T., Zhuang X. (2007). Multicolor super-resolution imaging with photoswitchable fluorescent probes. Science 317, 1749-1753 - PMC - PubMed
    1. Betzig E., Patterson G. H., Sougrat R., Lindwasser O. W., Olenych S., Bonifacino J. S., Davidson M. W., Lippincott-Schwartz J., Hess H. F. (2006). Imaging intracellular fluorescent proteins at nanometer resolution. Science 313, 1642-1645 - PubMed
    1. Bretschneider S., Eggeling C., Hell S. W. (2007). Breaking the diffraction barrier in fluorescence microscopy by optical shelving. Phys. Rev. Lett. 98, 218103 - PubMed
    1. Dellagiacoma C., Lukinavicius G., Bocchio N., Banala S., Geissbuhler S., Marki I., Johnsson K., Lasser T. (2010). Targeted photoswitchable probe for nanoscopy of biological structures. Chembiochem. 11, 1361-1363 - PubMed
    1. Ding J. B., Takasaki K. T., Sabatini B. L. (2009). Supraresolution imaging in brain slices using stimulated-emission depletion two-photon laser scanning microscopy. Neuron 63, 429-437 - PMC - PubMed

Publication types

MeSH terms