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. 2011 Jul;55(7):3588-90.
doi: 10.1128/AAC.01586-10. Epub 2011 May 2.

In vitro susceptibility of Pythium insidiosum to macrolides and tetracycline antibiotics

Erico Silva Loreto  1 , Débora Alves Nunes Mario, Laura Bedin Denardi, Sydney Hartz Alves, Janio Morais Santurio
Affiliations

In vitro susceptibility of Pythium insidiosum to macrolides and tetracycline antibiotics

Erico Silva Loreto et al. Antimicrob Agents Chemother. 2011 Jul.

Abstract

We describe the in vitro activity of macrolides and tetracycline antibiotics against Pythium insidiosum. The MICs were determined according to CLSI procedures (visual MIC) and by a colorimetric method [3-(4,5-dimethyl-2-thiazyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT)]. The lowest geometric mean (GM) MIC (MICs in μg/ml) (0.39 and 0.7 by visual reading and colorimetric method, respectively) and MIC ranges (0.125 to 2.0) were obtained for minocycline, while the highest MICs were shown for erythromycin (GM of 7.58 and 12.25 by visual reading and colorimetric method, respectively, and MIC ranged from 2 to 32). This significant in vitro activity makes these classes of antibiotics good candidates for experimental treatment of pythiosis.

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Figures

Fig. 1.
Fig. 1.
In vitro activity of azithromycin (rows A and B), clarithromycin (rows C and D), doxycycline (rows E and F), and erythromycin (rows G and H) against Pythium insidiosum after the inclusion of MTT. The yellow wells yellow indicate fungal inhibition, while hyphae are stained purple. The drug concentrations 10-fold serially diluted decreased from columns 1 (32 μg/ml) to 10 (0.06 μg/ml); column 11 is the negative control, and column 12 is the positive control (growth of P. insidiosum without drugs).
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References

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