Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2011:2011:218231.
doi: 10.1155/2011/218231. Epub 2011 Mar 30.

Chromosome mapping of repetitive sequences in Rachycentron canadum (Perciformes: Rachycentridae): implications for karyotypic evolution and perspectives for biotechnological uses

Uedson Pereira Jacobina  1 , Marcelo de Bello Cioffi, Luiz Gustavo Rodrigues Souza, Leonardo Luiz Calado, Manoel Tavares, João Manzella Jr, Luiz Antonio Carlos Bertollo, Wagner Franco Molina
Affiliations

Chromosome mapping of repetitive sequences in Rachycentron canadum (Perciformes: Rachycentridae): implications for karyotypic evolution and perspectives for biotechnological uses

Uedson Pereira Jacobina et al. J Biomed Biotechnol. 2011.

Abstract

The cobia, Rachycentron canadum, a species of marine fish, has been increasingly used in aquaculture worldwide. It is the only member of the family Rachycentridae (Perciformes) showing wide geographic distribution and phylogenetic patterns still not fully understood. In this study, the species was cytogenetically analyzed by different methodologies, including Ag-NOR and chromomycin A(3) (CMA(3))/DAPI staining, C-banding, early replication banding (RGB), and in situ fluorescent hybridization with probes for 18S and 5S ribosomal genes and for telomeric sequences (TTAGGG)(n). The results obtained allow a detailed chromosomal characterization of the Atlantic population. The chromosome diversification found in the karyotype of the cobia is apparently related to pericentric inversions, the main mechanism associated to the karyotypic evolution of Perciformes. The differential heterochromatin replication patterns found were in part associated to functional genes. Despite maintaining conservative chromosomal characteristics in relation to the basal pattern established for Perciformes, some chromosome pairs in the analyzed population exhibit markers that may be important for cytotaxonomic, population, and biodiversity studies as well as for monitoring the species in question.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Karyotypes of Rachycentron canadum. Conventional staining (a) highlighting the Ag-NOR sites in chromosome 2; (b) C-banding, highlighting the NORs as CMA3+/DAPI− heterochromatic regions; (c) replication bands, showing 18S (pair 2) and 5S (pairs 3 and 13) rDNA sites with early replication; (d) double-FISH with 18S (pink) and 5S (green) rDNA probes, showing the location of 18S rDNA sites in pair 2 and of 5S rDNA sites in pairs 3 and 13; (e) FISH with (TTAGGG)n sequences showing the location of telomeric sites in the chromosomes (orange). Bar = 5 μm.
Figure 2
Figure 2
Idiogram of the chromosome complement of Rachycentron canadum, exhibiting cytogenetic mapping of ribosomal sequences, Ag-NORs, heterochromatic regions, and chromosome replication bands. Bar = 5 μm.
See this image and copyright information in PMC

References

    1. Kaiser JB, Holt GJ. Cobia: a new species for aquaculture in the US. World Aquaculture Magazine. 2004;35:12–14.
    1. Liao IC, Huang TS, Tsai WS, Hsueh CM, Chang SL, Leaño EM. Cobia culture in Taiwan: current status and problems. Aquaculture. 2004;237(1–4):155–165.
    1. Benetti DD, Orhun MR, Zink I, et al. Aquaculture of cobia (Rachycentron canadum) in the Americas and the Caribbean. In: Liao IC, Leano EM, editors. Cobia Aquaculture: Research, Development and Commercial Production. New Orleans, La, USA: World Aquaculture Society; 2007. pp. 57–78.
    1. Benetti DD, O’Hanlon B, Rivera JA, Welch AW, Maxey C, Orhun MR. Growth rates of cobia (Rachycentron canadum) cultured in open ocean submerged cages in the Caribbean. Aquaculture. 2010;302(3-4):195–201.
    1. Frank JS, Ogle JT, Lotz JM, Nicholson LC, Barnes DN, Larsen KM. Spontaneous spawning of cobia, Rachycentron canadum, induced by human chorionic gonadotropin (HCG), with comments on fertilization, hatching and larval development. Proceedings of the Gulf and Caribbean Fisheries Institute. 2001;52:598–609.

Publication types

LinkOut - more resources