Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1990;153(2):134-8.
doi: 10.1007/BF00247810.

DNA topoisomerase I from Alcaligenes eutrophus H16

L Andĕra  1 , K Mikulík
Affiliations

DNA topoisomerase I from Alcaligenes eutrophus H16

L Andĕra et al. Arch Microbiol. 1990.

Abstract

Molecular and functional properties of DNA topoisomerase I isolated from a hydrogen-oxidizing bacterium, Alcaligenes eutrophus H16, were investigated. Under native conditions the enzyme forms a monomer with a relative molar mass of 98,500. A rod-like shape of the molecule was derived from the calculated frictional coefficient. The isoelectric point of the enzyme was determined to be in the range of 7.6-8.0. The enzyme activity is strictly Mg2+ dependent with an optimum at 3 mM Mg2+. The pH optimum ranges within 7.5-9.0. A. eutrophus DNA topoisomerase I activity is inhibited by M13 ssDNA, high ionic strength, polyamines, heparin and by a number of intercalating drugs.

PubMed Disclaimer

References

    1. J Biol Chem. 1977 Feb 10;252(3):1102-6 - PubMed
    1. J Bacteriol. 1986 Nov;168(2):636-41 - PubMed
    1. Biochim Biophys Acta. 1987 Jun 6;909(1):1-9 - PubMed
    1. Proc Natl Acad Sci U S A. 1978 Sep;75(9):4097-101 - PubMed
    1. Biochem J. 1987 Jan 1;241(1):111-9 - PubMed

MeSH terms

LinkOut - more resources