Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2008;35(3):228-238.
doi: 10.1159/000124281. Epub 2008 May 8.

In vitro Differentiation Potential of Mesenchymal Stem Cells

Jeffrey M Gimble  1 , Farshid Guilak, Mark E Nuttall, Solomon Sathishkumar, Martin Vidal, Bruce A Bunnell
Affiliations

In vitro Differentiation Potential of Mesenchymal Stem Cells

Jeffrey M Gimble et al. Transfus Med Hemother. 2008.

Abstract
in English, German

SUMMARY: Mesenchymal stem cells (MSCs) represent a class of multipotent progenitor cells that have been isolated from multiple tissue sites. Of these, adipose tissue and bone marrow offer advantages in terms of access, abundance, and the extent of their documentation in the literature. This review focuses on the in vitro differentiation capability of cells derived from adult human tissue. Multiple, independent studies have demonstrated that MSCs can commit to mesodermal (adipocyte, chondrocyte, hematopoietic support, myocyte, osteoblast, tenocyte), ectodermal (epithelial, glial, neural), and endodermal (hepatocyte, islet cell) lineages. The limitations and promises of these studies in the context of tissue engineering are discussed.

Zusammenfassung: Mesenchymale Stammzellen (MSCs) sind mulipotente Vorläuferzellen, die bisher aus verschiedenen Geweben isoliert wurden. Fettgewebe und Knochenmark gelten bezüglich des Gewebezugangs, der Stammzellmengen und ihrer Dokumentation in der wissenschaftlichen Literatur als besonders vorteilhaft. Schwerpunkt dieser Literaturzusammenfassung ist die In-vitro-Differenzierungs-fähigkeit menschlicher Gewebezellen. Mehrere unabhängige Studien berichten, dass MSCs zur Differenzierung in sowohl mesenchymale (Adipozyten, Chondrozyten, hämatopoetischen Unterstützungzellen, Myozyten, Osteoblasten, Tenozyten) als auch ektodermale (epitheliale, gliale, and neurale Zellen) als auch endodermale (Hepatozyten, Pankreasinselzellen) Zelllinien fähig sind. Die Limitationen und Aussichten dieser Studien im Kontext von Tissue Engineering werden diskutiert.

PubMed Disclaimer

References

    1. Friedenstein AJ. Precursor cells of mechanocytes. Int Rev Cytol. 1976;47:327–359. - PubMed
    1. Friedenstein AJ, Chailakhjan RK, Lalykina KS. The development of fibroblast colonies in monolayer cultures of guinea-pig bone marrow and spleen cells. Cell Tissue Kinet. 1970;3:393–403. - PubMed
    1. Owen M. Marrow stromal stem cells. J Cell Sci Suppl. 1988;10:63–76. - PubMed
    1. Tavassoli M, Crosby WH. Bone marrow histogenesis: a comparison of fatty and red marrow. Science. 1970;169:291–293. - PubMed
    1. Friedenstein AJ, Lalykina KS. Thymus cells are inducible to osteogenesis. Eur J Immunol. 1972;2:602–603. - PubMed