Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2008;35(3):239-247.
doi: 10.1159/000129129. Epub 2008 May 16.

In vivo Differentiation Potential of Mesenchymal Stem Cells: Prenatal and Postnatal Model Systems

Courtney Quinn  1 , Alan W Flake
Affiliations

In vivo Differentiation Potential of Mesenchymal Stem Cells: Prenatal and Postnatal Model Systems

Courtney Quinn et al. Transfus Med Hemother. 2008.

Abstract
in English, German

SUMMARY: Most of our knowledge of mesenchymal stem cell (MSC) biology is derived from in vitro systems that are often highly contrived to favor culture expansion or specific differentiation events. However, any conclusions drawn from in vitro studies regarding MSC differentiation capacity, immune properties, or therapeutic potential must be validated by in vivo studies to ultimately be meaningful. At the present time, there are relatively few in vivo studies demonstrating differentiation and functional integration of MSCs into host tissues after transplantation. There is a need for in vivo model systems to assay MSC biology and to move potential therapeutic strategies forward. Here, we review prenatal model systems as potentially advantageous for the in vivo characterization of MSCs, and we critically review the results of in vivo studies of MSC transplantation in prenatal and postnatal model systems with an emphasis on proven engraftment and differentiation.

Zusammenfassung: Unser Wissen über die Biologie mesenchymaler Stammzellen (MSCs) basiert hauptsächlich auf In-vitro-Systemen, welche oft auf sehr künstliche Art und Weise das Kulturwachstum oder spezifische Differenzierungs-vorgänge begünstigen. Aus In-vitro-Studien gezogene Schlussfolgerungen bezüglich der Differenzierungskapazität, der Immuneigenschaften oder des therapeutischen Potentials von MSCs müssen jedoch durch In-vivo-Studien bestätigt werden, um aussagekräftig sein zu können. Im Moment gibt es relativ wenige In-vivo-Studien, die die Differenzierung und funktionelle Integration von MSCs in das Gewebe des Empfängers nach einer Transplantation demonstrieren. Es besteht Bedarf an ln-vivo-Modellsystemen, mit denen die MSC-Biologie geprüft und potentielle therapeutische Strategien gefördert werden können. Wir geben einen Überblick über pränatale Modellsysteme, die für die In-vivo-Charakterisierung von MSCs potentiell von Vorteil sein könnten, und bewerten kritisch die Ergebnisse von In-vivo-Studien zur Transplantation von MSCs in prä- und postnatalen Modellsystemen mit Betonung auf nachgewiesenem Anwachsen und Differenzierung der Zellen.

PubMed Disclaimer

References

    1. Johnstone B, Hering TM, Caplan AI, et al. In vitro chondrogenesis of bone marrow-derived mesenchymal progenitor cells. Exp Cell Research. 1998;238:265–72. - PubMed
    1. Le Blanc K, Tammik L, Sundberg B, et al. Mesechymal stem cells inhibit and stimulate mixed lymphocyte cultures and mitogenic responses independently of the major histocompatibility complex. Scand J Immunol. 2003;57:11–20. - PubMed
    1. Muller I, Kustermann-Kuhn B, Holzwarth C, et al. In vitro analysis of multipotent mesenchymal stremal cells as potential cellular therapeutics in neu-rometabolic disesases in pediatric patients. Exp Hematol. 2006;34:1413–19. - PubMed
    1. Rieger K, Marinets O, Fietz T, et al. Mesenchymal stem cells remain of host origin even a long time after allogeneic peripheral blood stem cell or bone marrow transplantation. Exp Hematol. 2005;33:605–11. - PubMed
    1. Wang J, Liu K, Lu DP. Mesenchymal stem cells in stem cell transplant recipients are damaged and remain of host origin. Int J Hematol. 2005;82:152–8. - PubMed