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. 2011 Apr 3:2011:304349.
doi: 10.4061/2011/304349.

Comparative Study of Serological Tests for Mycoplasma synoviae Diagnosis in Commercial Poultry Breeders

R L Luciano  1 , A L S P Cardoso, G F Z Stoppa, A M I Kanashiro, A G M de Castro, E N C Tessari
Affiliations

Comparative Study of Serological Tests for Mycoplasma synoviae Diagnosis in Commercial Poultry Breeders

R L Luciano et al. Vet Med Int. .

Abstract

Avian mycoplasmosis causes great economic losses to the poultry industry, and one of the major agents involved is Mycoplasma synovie (MS). Serum from commercial poultry breeders (n = 2781) was tested for MS by serum plate agglutination (SPA), hemagglutination inhibition (HI), and enzyme-linked immunosorbent assay (ELISA). From 2,781 samples tested, 736 (26.46%) were positive in SPA. From 712 SPA-positive sera, 30 samples (4.21%) were positive in HI, and 150 samples (21.06%) were positive in ELISA. Copositivity between ELISA and HI was 90%, and conegativity was 82.0%. Agreement between HI and ELISA was rejected by McNemar's test (P ≤ .001), and Kappa coefficient showed a weak correlation between the two techniques (k = 0.25; 0.21 ≤ k < 0.40). Weak statistical correlation was observed between all serological tests (SPA, HI, and ELISA), and they should only be used for initial screening for MS.

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