Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1990 Jan;16(1):29-38.
doi: 10.1007/BF01650477.

SV40-mediated tumor selection and chromosome transfer to enrich for cystic fibrosis region

D J Porteous  1 , J R Dorin, M M Wilkinson, J M Fletcher, E Emslie, V van Heyningen
Affiliations

SV40-mediated tumor selection and chromosome transfer to enrich for cystic fibrosis region

D J Porteous et al. Somat Cell Mol Genet. 1990 Jan.

Abstract

The somatic cell hybrid C121, with chromosome 7 as its sole human component, arose when mouse macrophages SV40 genomes are integrated at 7q31-7q35. We show that hybrids with a reduced chromosome 7 component, but which retain markers linked to the cystic fibrosis locus, can be generated by direct in vivo tumor selection or following chromosome-mediated gene transfer and SV40-mediated cellular transformation. Our methods for chromosome fragmentation and fine-structure mapping can now be applied to the substantial number of SV40-transformed human cell lines, with independent chromosomal integration sites, already available. Our results also suggest that expression of human epidermal growth factor receptor augments the tumorigenic potential of the SV40-transformed C121 hybrid.

PubMed Disclaimer

Publication types