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. 1990 Feb 26;261(2):431-5.
doi: 10.1016/0014-5793(90)80609-m.

Are there isoenzymes of cytochrome c oxidase in Paracoccus denitrificans?

M Raitio  1 , J M Pispa, T Metso, M Saraste
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Free article

Are there isoenzymes of cytochrome c oxidase in Paracoccus denitrificans?

M Raitio et al. FEBS Lett. .
Free article

Abstract

We have used a gene replacement strategy to delete the previously isolated gene [(1987) EMBO J. 6, 2825-2833] for the cytochrome c oxidase subunit I from Paracoccus denitrificans. The resulting mutant was still able to synthesize active cytochrome c oxidase. This led us to look for another locus which could completely suppress the mutation. In this study we report the isolation of a second gene encoding subunit I. An open reading frame coding for cytochrome c 550 was found upstream from this gene. We suggest that there are isoenzymes of cytochrome c oxidase (cytochrome aa3) in this bacterium.

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