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. 1990 Apr;172(4):2181-3.
doi: 10.1128/jb.172.4.2181-2183.1990.

Isolation of a Rhodobacter capsulatus bioB mutant and cloning of the bioB gene

R D Cardin  1 , A J Biel
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Isolation of a Rhodobacter capsulatus bioB mutant and cloning of the bioB gene

R D Cardin et al. J Bacteriol. 1990 Apr.

Abstract

We isolated a Tn5 insertion mutant of Rhodobacter capsulatus which requires biotin for growth. Crossfeeding studies with Escherichia coli bio mutants indicated that the insertion is in the bioB gene. A cosmid that complements the bioB insertion was isolated, and the bioB gene was localized to a 2.85-kilobase EcoRI-PstI fragment.

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