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. 2011 Jul;156(3):1331-6.
doi: 10.1104/pp.111.177873. Epub 2011 May 12.

The role of brassinosteroids in shoot gravitropism

Filip Vandenbussche  1 , Dmitry Suslov, Liesbeth De Grauwe, Olivier Leroux, Kris Vissenberg, Dominique Van der Straeten
Affiliations

The role of brassinosteroids in shoot gravitropism

Filip Vandenbussche et al. Plant Physiol. 2011 Jul.
No abstract available

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Figures

Figure 1.
Figure 1.
BRs and Suc differentially affect gravitropic growth of Arabidopsis seedlings. A, Three-day-old etiolated seedlings grown on one-half-strength Murashige and Skoog medium with or without 1% Suc and different concentrations of 24-EBL. B, Bar graph representing the number of plants that are standing (nonhorizontal) when grown as in A. Values are means of at least four independent biological experiments. Error bars are sem. a, Statistically different from non-EBL-treated seedlings (Student’s t test, P < 0.05); b, statistically different from seedlings grown without exogenous Suc (Student’s t test, P < 0.05). C, Effect of 1% Glc and 1% mannitol on the BR response. Values are means of at least four independent biological experiments. Error bars are sem. a, Statistically different from the respective, non-EBL-treated counterpart (Student’s t test, P < 0.05); b, statistically different from the respective counterpart grown without exogenous sugar (Student’s t test, P < 0.05).
Figure 2.
Figure 2.
BR deficiency positively affects gravitropism independently of the presence of starch-containing statoliths. A and B, Bar graphs representing the number of 3-d-old etiolated wild-type and mutant seedlings standing (nonhorizontal) in the presence or absence of 2 μm Brz and/or 1% Suc. Values are means of at least four independent biological experiments. Error bars are sem. Asterisks (**) indicate values statistically different from the non-Brz-treated counterpart (based on Student’s t test, P < 0.05); asterisk (*) indicates values statistically different from the non-Suc-treated counterpart (based on Student’s t test, P < 0.05). A, Wild-type Col-0 versus pgm mutants. B, Wild-type Wassilewskija versus scr mutants. C, Lugol stain of etiolated Col-0 wild-type and pgm mutant seedlings grown in the presence or absence of 1% Suc and/or combined with either 2 μm Brz or 100 nm 24-EBL. The top portion of the hypocotyl is shown. Photographs were taken at the same magnification. Bars = 100 μm. [See online article for color version of this figure.]
Figure 3.
Figure 3.
Cell wall mechanics of frozen/thawed hypocotyls of dark-grown seedlings in various conditions. A, In vitro extension (expressed as creep rate % h−1) of hypocotyls from 4-d-old wild-type Col-0 plants grown without Suc (0% Suc) ± 100 nm 24-EBL and from 5-d-old plants grown with 1% Suc ± 100 nm EBL. Error bars represent sem (n ≥ 10). B, In vitro extension of hypocotyls from 5-d-old wild-type Col-0 (wt) and pgm plants grown with 1% Suc. Error bars represent sem (n = 8).
Figure 4.
Figure 4.
Response of wild-type Col-0 seedlings after growth and reorientation on vertically standing plates. Seedlings were grown in darkness. On day 2 after germination, plates were rotated and the average reorientation angle of the hypocotyl was calculated. Data are mean values of at least five seedlings. Seedlings were grown on one-half-strength Murashige and Skoog media without (control) or with 1% Suc (1%suc), 2 μm Brz, or 100 nm 24-EBL. 90° corresponds with the new direction of the gravity vector.
See this image and copyright information in PMC

References

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