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. 2011 May 15;7(7):425-7.
doi: 10.1038/nchembio.577.

A universal code for RNA recognition by PUF proteins

Aleksandra Filipovska  1 , Muhammad F M Razif, Karoline K A Nygård, Oliver Rackham
Affiliations

A universal code for RNA recognition by PUF proteins

Aleksandra Filipovska et al. Nat Chem Biol. .

Abstract

The design of proteins that can bind any RNA sequence of interest has many potential biological and medical applications. Here we have expanded the recognition of Pumilio and FBF homology protein (PUF) repeats beyond adenine, guanine and uracil and evolved them to specifically bind cytosine. These repeat sequences can be used to create PUF domains capable of selectively binding RNA targets of diverse sequence and structure.

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References

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