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. 2011 Jul;63(1):87-93.
doi: 10.1007/s00284-011-9949-6. Epub 2011 May 15.

Genetic diversity of Pasteurella dagmatis as assessed by analysis of the 16S rRNA and rpoB gene sequences

Jarosław Król  1 , Jacek Bania, Magdalena Florek, Magdalena Podkowik, Aleksandra Pliszczak-Król, Zdzisław Staroniewicz
Affiliations

Genetic diversity of Pasteurella dagmatis as assessed by analysis of the 16S rRNA and rpoB gene sequences

Jarosław Król et al. Curr Microbiol. 2011 Jul.

Abstract

A total of 16 Pasteurella dagmatis strains, including 11 feline and 4 canine isolates as well as one strain isolated from a tiger, were analyzed using partial 16S rRNA and rpoB gene sequence comparison. Phylogenetic studies based on both genes revealed that the population of P. dagmatis recovered from cats in Poland differs markedly from canine strains, constituting a well-separated cluster within Pasteurella sensu stricto species group. The isolate from a tiger seems to represent yet another evolutionary lineage within P. dagmatis.

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Figures

Fig. 1
Fig. 1
Phylogenetic tree of 36 Pasteurella strains based on 16S rRNA sequences (1308 bp fragments). The tree was constructed using the neighbor-joining method. Bootstrap values of 500 replications are indicated as percent confidence values for particular branching. Sequences indicated in bold were determined in this study. CCUG Culture Collection, University of Göteborg, Sweden; NCTC National Collection of Type Cultures, UK; MCCM Medical Culture Collection Marburg, Germany; ATCC American Type Culture Collection, USA; CNP Centre National des Pasteurella, France; CDC Centers for Disease Control and Prevention, USA; T type strain; (F) isolates from cats; (C) isolates from dogs; (T) the isolate from a tiger
Fig. 2
Fig. 2
Phylogenetic tree of 28 Pasteurella strains based on rpoB sequences (501 bp fragments). The tree was constructed using the neighbor-joining method. Bootstrap values of 500 replications are indicated as percent confidence values for particular branching. Sequences indicated in bold were determined in this study. CCUG Culture Collection, University of Göteborg, Sweden; NCTC National Collection of Type Cultures, UK; CDC Centers for Disease Control and Prevention, USA; T type strain; (F) isolates from cats; (C) isolates from dogs; (T) the isolate from a tiger
Fig. 3
Fig. 3
Agarose gel electrophoresis of a 476-bp fragment of the P. dagmatis 16S rRNA gene. Lanes 1–8 represent amplicons obtained from DNA of canine isolates, lanes 9–16 from DNA of feline isolates, and lanes 17–18 from DNA of the isolate from a tiger. For each isolate, intact amplicons (lanes with odd numbers) and TaqI-digested ones (lanes with even numbers) were run. M size marker (GeneRuler™100 bp DNA Ladder [Fermentas])
See this image and copyright information in PMC

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