Effects of 5' and 3' truncations of the myb gene on the transforming ability of avian myeloblastosis virus (AMV)

Abstract

Proviruses based on the avian myeloblastosis virus (AMV) have been constructed which code for variations of the c-myb and/or v-myb gene product. These proviruses have been used in a soft colony agar assay to assess the contributions of the 5' and 3' deletions of the v-myb oncogene in the cellular transforming activity of the virus. The results indicate that 3' truncations are an integral part of the gene's mechanism of activation and that the truncations on the 5' end of the gene are important either in its mechanism of activation or its expression by viral control elements.