Mutations in polyomavirus large T affecting immortalization of primary rat embryo fibroblasts
- PMID: 2158701
- DOI: 10.1016/0042-6822(90)90234-i
Mutations in polyomavirus large T affecting immortalization of primary rat embryo fibroblasts
Abstract
To clarify the relationship between various functions of the polyomavirus large T antigen and the contribution of this oncogene toward neoplastic transformation, we have analyzed the properties of mutants with in-frame deletions in the second large T exon. dl45, dl96, and dl97 have retained the ability to immortalize primary rat embryo fibroblasts and to trans-activate viral promoters. dl8, dl23, and dl300, which are deficient immortalization, are also deficient in transactivation. However, a newly constructed mutant, designated dl141, which is deficient in immortalization, is still able to trans-activate both the polyoma and SV40 late promoters. This indicates that the ability to trans-activate promoters is not sufficient to confer on the large T antigen the ability to immortalize primary cells.
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