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. 2011 Jul;49(7):2697-9.
doi: 10.1128/JCM.00336-11. Epub 2011 May 18.

Genotypic prediction of human immunodeficiency virus type 1 tropism by use of plasma and peripheral blood mononuclear cells in the routine clinical laboratory

Christian Paar  1 , Maria Geit, Herbert Stekel, Jörg Berg
Affiliations

Genotypic prediction of human immunodeficiency virus type 1 tropism by use of plasma and peripheral blood mononuclear cells in the routine clinical laboratory

Christian Paar et al. J Clin Microbiol. 2011 Jul.

Abstract

We developed a sequencing assay for genotypic HIV-1 tropism determination. The assay allows examination of HIV RNA from plasma and HIV DNA from peripheral blood mononuclear cells (PBMC), including PBMC samples from patients with undetectable viral loads. Assessment of 100 pairs of plasma and PBMC samples showed a high concordance of 90%. With the limitations of population-based sequencing, the assay was found to be robust and suitable for the routine clinical laboratory.

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References

    1. Berger E. A., et al. 1998. A new classification for HIV-1. Nature 391:240. - PubMed
    1. Coakley E., et al. 2009. Comparison of human immunodeficiency virus type 1 tropism profiles in clinical samples by the Trofile and MT-2 assays. Antimicrob. Agents Chemother. 53:4686–4693 - PMC - PubMed
    1. Corbeau P., Reynes J. 2009. CCR5 antagonism in HIV infection: ways, effects, and side effects. AIDS 23:1931–1943 - PubMed
    1. Delobel P., et al. 2007. Population-based sequencing of the V3 region or env for predicting the coreceptor usage of human immunodeficiency virus type 1 quasispecies. J. Clin. Microbiol. 45:1572–1580 - PMC - PubMed
    1. Dorr P., et al. 2005. Maraviroc (UK-427,857), a potent, orally bioavailable, and selective small-molecule inhibitor of chemokine receptor CCR5 with broad-spectrum anti-human immunodeficiency virus type 1 activity. Antimicrob. Agents Chemother. 49:4721–4732 - PMC - PubMed

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