Widespread RNA and DNA sequence differences in the human transcriptome
- PMID: 21596952
- PMCID: PMC3204392
- DOI: 10.1126/science.1207018
Widespread RNA and DNA sequence differences in the human transcriptome
Abstract
The transmission of information from DNA to RNA is a critical process. We compared RNA sequences from human B cells of 27 individuals to the corresponding DNA sequences from the same individuals and uncovered more than 10,000 exonic sites where the RNA sequences do not match that of the DNA. All 12 possible categories of discordances were observed. These differences were nonrandom as many sites were found in multiple individuals and in different cell types, including primary skin cells and brain tissues. Using mass spectrometry, we detected peptides that are translated from the discordant RNA sequences and thus do not correspond exactly to the DNA sequences. These widespread RNA-DNA differences in the human transcriptome provide a yet unexplored aspect of genome variation.
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Comment in
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Widespread promiscuous genetic information transfer from DNA to RNA.Circ Res. 2011 Nov 11;109(11):1202-3. doi: 10.1161/RES.0b013e31823c4992. Circ Res. 2011. PMID: 22076505 No abstract available.
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Comment on "Widespread RNA and DNA sequence differences in the human transcriptome".Science. 2012 Mar 16;335(6074):1302; author reply 1302. doi: 10.1126/science.1209658. Science. 2012. PMID: 22422962
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Comment on "Widespread RNA and DNA sequence differences in the human transcriptome".Science. 2012 Mar 16;335(6074):1302; author reply 1302. doi: 10.1126/science.1210484. Science. 2012. PMID: 22422963 Free PMC article.
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Comment on "Widespread RNA and DNA sequence differences in the human transcriptome".Science. 2012 Mar 16;335(6074):1302; author reply 1302. doi: 10.1126/science.1210419. Science. 2012. PMID: 22422964
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RNA-DNA sequence differences spell genetic code ambiguities.Artif DNA PNA XNA. 2011 Jul-Dec;2(3):69-70. doi: 10.4161/adna.17086. Artif DNA PNA XNA. 2011. PMID: 22567189 Free PMC article.
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