Characterization of Mesorhizobium loti L-rhamnose isomerase and its application to L-talose production
- PMID: 21597169
- DOI: 10.1271/bbb.110018
Characterization of Mesorhizobium loti L-rhamnose isomerase and its application to L-talose production
Abstract
The L-rhamnose isomerase gene (rhi) of Mesorhizobium loti was cloned and expressed in Escherichia coli, and then characterized. The enzyme exhibited activity with respect to various aldoses, including D-allose and L-talose. Application of it in L-talose production from galactitol was achieved by a two-step reaction, indicating that it can be utilized in the large-scale production of L-talose.
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