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. 2011;75(5):1006-9.
doi: 10.1271/bbb.110018. Epub 2011 May 20.

Characterization of Mesorhizobium loti L-rhamnose isomerase and its application to L-talose production

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Characterization of Mesorhizobium loti L-rhamnose isomerase and its application to L-talose production

Goro Takata et al. Biosci Biotechnol Biochem. 2011.
Free article

Abstract

The L-rhamnose isomerase gene (rhi) of Mesorhizobium loti was cloned and expressed in Escherichia coli, and then characterized. The enzyme exhibited activity with respect to various aldoses, including D-allose and L-talose. Application of it in L-talose production from galactitol was achieved by a two-step reaction, indicating that it can be utilized in the large-scale production of L-talose.

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