Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2011 Oct;31(7):1071-8.
doi: 10.1007/s10571-011-9706-1. Epub 2011 May 20.

p65/RelA-Ser529 NF-κB subunit phosphorylation induces autophagic astroglial death (Clasmatodendrosis) following status epilepticus

Hea Jin Ryu  1 , Ji-Eun Kim, Seong-Il Yeo, Tae-Cheon Kang
Affiliations

p65/RelA-Ser529 NF-κB subunit phosphorylation induces autophagic astroglial death (Clasmatodendrosis) following status epilepticus

Hea Jin Ryu et al. Cell Mol Neurobiol. 2011 Oct.

Abstract

Clasmatodendrosis is an irreversible astroglial degenerative change, which includes extensive swelling and vacuolization of cell bodies and disintegrated and beaded processes. This study was designed to elucidate whether clasmatodendrosis may be one of the autophagy-related degeneration of astrocytes. In this study, clasmatodendritic astrocytes were observed only in the stratum radiatum in the CA1 region. Vacuoles in clasmatodendritic astrocytes showed LAMP-1 immunoreactivity. In addition, both LC3-II and Beclin-1 expression were detected in most of clasmatodendritic astrocytes as well as a few non-vacuolized astrocytes. Clasmatodendritic astrocytes also showed p65/RelA-Ser529 phosphorylation in the nuclei. The neutralization of TNF-α by sTNFp55R infusion reduced clasmatodendritic astrocytes with nuclear p65/RelA-Ser529 phosphorylation. Therefore, these findings suggest that clasmatodendrosis may be autophagic astroglial death in response to epileptic seizures through TNF-α-mediated p65/RelA-Ser529 phosphorylation.

PubMed Disclaimer

Figures

Fig. 1
Fig. 1
Clasmatodendorosis is autophagic astroglial death. (a) Vacuoles in clasmatodendritic astrocytes show LAMP-1 immunoreactivity (arrows). (b) Vacuoles in clasmatodendritic astrocytes do not contain EEA-1 immunoreactivity (arrowheads). (c) Beclin-1 immunoreactivity is observed in the cytoplasm of clasmatodenritic astrocytes (arrows). (d) Vacuoles in clasmatodendritic astrocytes show LC3-II immunoreactivity (arrows). Bar = 12.5 μm
Fig. 2
Fig. 2
p65/RelA NF-κB phosphorylation and TNFp75 receptor expression in clasmatodendritic astrocytes. (a) p65/RelA-Ser276 NF-κB phosphorylation. (b) p65/RelA-Ser311 NF-κB phosphorylation. (c) p65/RelA-Ser529 NF-κB phosphorylation. Only p65/RelA-Ser529 NF-κB phosphorylation is observed in the watery nucleus of clasmatodendritic astrocytes (arrow). (d) TNFp75 receptor expression in clasmatodendritic astrocytes (arrows). Bar = 5 (c) and 10 (a, b, and d) μm
Fig. 3
Fig. 3
Effect of sTNFp55R infusion on clasmatodendrosis (a, b). As compared with saline-infusion (a, arrows), sTNFp55R infusion reduces the fraction of clasmatodendritic astrocytes in total astrocytes, and accumulation of nuclear p65/RelA-Ser529 phosphorylation (b, arrows). Bar = 15 μm. (c) The quantitative analysis of the fraction of clasmatodendritic astrocytes in the total astrocytes (mean ± SEM). Significant differences from saline-infused animals, *P < 0.05
See this image and copyright information in PMC

References

    1. Aita VM, Liang XH, Murty VV, Pincus DL, Yu W, Cayanis E, Kalachikov S, Gilliam TC, Levine B (1999) Cloning and genomic organization of beclin 1, a candidate tumor suppressor gene on chromosome 17q21. Genomics 59:59–65 - PubMed
    1. Bedi KS (1991) Early-life undernutrition causes deficits in rat dentate gyrus granule cell number. Experientia 47:1073–1074 - PubMed
    1. Ben Achour S, Pascual O (2010) Glia: the many ways to modulate synaptic plasticity. Neurochem Int 57:440–445 - PubMed
    1. Budagian V, Bulanova E, Brovko L, Orinska Z, Fayad R, Paus R, Bulfone-Paus S (2003) Signaling through P2X7 receptor in human T cells involves p56lck, MAP kinases, and transcription factors AP-1 and NF-kappa B. J Biol Chem 278:549–560 - PubMed
    1. Bursch W, Ellinger A, Kienzl H, Török L, Pandey S, Sikorska M, Walker R, Hermann RS (1996) Active cell death induced by the anti-estrogens tamoxifen and ICI 164 384 in human mammary carcinoma cells (MCF-7) in culture: the role of autophagy. Carcinogenesis 17:1595–1607 - PubMed

Publication types

Substances

LinkOut - more resources