Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2011 Jun;37(6):900-8.
doi: 10.1016/j.ultrasmedbio.2011.03.011.

Validation of dynamic contrast-enhanced ultrasound in rodent kidneys as an absolute quantitative method for measuring blood perfusion

Paul Kogan  1 , Kennita A Johnson, Steven Feingold, Nicholas Garrett, Ismayil Guracar, William J Arendshorst, Paul A Dayton
Affiliations

Validation of dynamic contrast-enhanced ultrasound in rodent kidneys as an absolute quantitative method for measuring blood perfusion

Paul Kogan et al. Ultrasound Med Biol. 2011 Jun.

Abstract

Contrast-enhanced ultrasound (CEUS) has demonstrated utility in the monitoring of blood flow in tissues, organs and tumors. However, current CEUS methods typically provide only relative image-derived measurements, rather than quantitative values of blood flow in milliliters/minute per gram of tissue. In this study, CEUS derived parameters of blood flow are compared with absolute measurements of blood flow in rodent kidneys. Additionally, the effects of contrast agent infusion rate and transducer orientation on image-derived perfusion measurements are assessed. Both wash-in curve and time-to-refill algorithms are examined. Data illustrate that for all conditions, image-derived flow measurements were well-correlated with transit-time flow probe measurements (R > 0.9). However, we report differences in the sensitivity to flow across different transducer orientations as well as the contrast analysis algorithm utilized. Results also indicate that there exists a range of contrast agent flow rates for which image-derived estimates are consistent.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Representative user selected ROI for a) cortial, b)outer medullary and c) inner medullary regions of the kidney for the coronal (left) and parasagittal (right). The MCA/KW infusion rate for these images was 23.0 μl/g·min.
Figure 2
Figure 2
A representative perfusion map using parametric method for one animal with decreasing renal blood flow (top to bottom). Green indicates faster flow and red indicates slower flow. A-C is the coronal orientation and the D-F is the parasagittal orientation. The MCA/KW infusion rate for these images was 34.0 μl/g·min. In order to reduce the RBF, the animal was given 0, 6 and 10 ng of AngII.
Figure 3
Figure 3
A representative plot of reperfusion rate (RR) vs. RBF for a single orientation and single MCA infusion rate/KW for both young (formula image, less than 500g) and old (●, more than 500g) rats.. The MCA/KW infusion rate for this curve is 31.1 μl/g·min in the coronal orientation.
Figure 4
Figure 4
A representative intensity vs. time curve for the cortical(▲), outer (+) and inner (*) medullary regions of a single kidney. The lines are curve fits to the equation y = A(1 – e-βt). The MCA/KW infusion rate for this curve is 59.4 μl/g·min in the coronal orientation with 0ng of AngII given to the animal.
Figure 5
Figure 5
A representative plot of β vs. RBF for a single orientation and single MCA infusion rate/KW for both young (formula image, less than 500g) and old (●, more than 500g) rats. The MCA/KW infusion rate for this curve is 52.8 μl/g·min in the coronal orientation.
Figure 6
Figure 6
Kidney weight (KW) vs. body weight (BW) for rats used in this study. Body weights ranged from 350g – 750g. This conversion can be used to convert from body weight to kidney weight in future non-invasive studies.
Figure 7
Figure 7
A plot of the slopes for the RBF vs. either reperfusion rate or β for different mean MCA infusion rates/KW.
See this image and copyright information in PMC

References

    1. Arendshorst W, Navar L. Renal Circulation and Glomerular Hemodyamics. In: Schrier, editor. Diseases of the Kidney and Urinary Tract. Lippincott Williams & Wilkins; Philadelphia: 2007.
    1. Chen NG, Fowlkes JB, Carson PL, Schipper MJ, LeCarpentier GL. Rapid 3-D imaging of contrast flow: application in a perfused kidney phantom. Ultrasound Med Biol. 2009;35:813–28. - PMC - PubMed
    1. Chomas JE, Pollard RE, Sadlowski AR, Griffey SM, Wisner ER, Ferrara KW. Contrast-enhanced US of microcirculation of superficially implanted tumors in rats. Radiology. 2003;229:439–46. - PubMed
    1. Cosgrove D, Lassau N. Imaging of perfusion using ultrasound. Eur J Nucl Med Mol Imaging. 2010;37(Suppl 1):S65–85. - PubMed
    1. Feingold S, Gessner R, Guracar IM, Dayton PA. Quantitative volumetric perfusion mapping of the microvasculature using contrast ultrasound. Invest Radiol. 2010;45:669–74. - PMC - PubMed

Publication types