Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1978 Dec;75(12):6125-9.
doi: 10.1073/pnas.75.12.6125.

Detection of viral sequences of low reiteration frequency by in situ hybridization

M Brahic, A T Haase

Detection of viral sequences of low reiteration frequency by in situ hybridization

M Brahic et al. Proc Natl Acad Sci U S A. 1978 Dec.

Abstract

The sensitivity of in situ hybridization has been increased at least 10-fold by hybridizing in cDNA excess, by increasing the diffusion of the cDNA through the cells, by hybridizing at optimum temperature, and by stabilizing hybrids during autoradiography. Saturation of intracellular RNA with [3H]cDNA has been achieved. The assay is quantitative. In situ hybridization has been used to detect and quantitate visna virus RNA in infected cells. By using [3H]cDNA with specific activity of 2 X 10(8) dpm/micrograms and conditions that reduce background to negligible levels, 10--20 copies of viral RNA per cell can be detected and quantitated after 2 days of autoradiographic exposure.

PubMed Disclaimer

References

    1. J Biol Chem. 1976 Dec 10;251(23):7355-62 - PubMed
    1. Biochem Biophys Res Commun. 1966 Jun 13;23(5):641-6 - PubMed
    1. Pathol Biol (Paris). 1977 Jun;25(6):365-8 - PubMed
    1. Science. 1977 Jan 14;195(4274):175-7 - PubMed
    1. J Mol Biol. 1977 Sep 25;115(3):539-63 - PubMed

Publication types

LinkOut - more resources