Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2011 May;31(5):849-53.

[Isolation and identification of stem cells derived from human exfoliated deciduous teeth by magnetic activated cell sorting]

[Article in Chinese]
Xiang-long Ding  1 , Ke Chen, Yuan-yuan Shen
Affiliations
  • PMID: 21602140

[Isolation and identification of stem cells derived from human exfoliated deciduous teeth by magnetic activated cell sorting]

[Article in Chinese]
Xiang-long Ding et al. Nan Fang Yi Ke Da Xue Xue Bao. 2011 May.

Abstract

Objective: To isolate stem cells from human exfoliated deciduous teeth (SHEDs) and identify their phenotypes and multi-lineage differentiation potential.

Methods: Human pulp tissue from exfoliated deciduous teeth were dissected and digested to obtain the single cell suspension. The SHEDs selected by magnetic activated cell sorting system (MACS) were identified by examination of the cell morphology and growth in vitro and detection of the expressions of the cell markers. Osteogenic and adipogenic induction was performed to test the multi-lineage differentiation potential of the cells.

Results: SHEDs were successfully isolated from human exfoliated deciduous teeth. SHEDs showed a lower growth rate than dental pulp cells and displayed high expressions of CD29 and CD105 but low expressions of CD34 and CD45 as shown by flow cytometry. Experiments of in vitro induction demonstrated a strong potential of the STRO-1+ SHEDs for osteogenic and adipogenic differentiation.

Conclusion: Immunomagnetic bead selection can be used to isolate and purify SHEDs, and the STRO-1+ SHEDs show the characteristics of stem cells with multipotent differentiation potentials.

PubMed Disclaimer

Publication types