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. 2011 Mar 3:2:6.
doi: 10.3389/fphys.2011.00006. eCollection 2011.

Ussing's "Little Chamber": 60 Years+ Old and Counting

Kirk L Hamilton  1
Affiliations

Ussing's "Little Chamber": 60 Years+ Old and Counting

Kirk L Hamilton. Front Physiol. .
No abstract available

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Figures

Figure 1
Figure 1
Diagram of the Ussing chamber apparatus. Abbreviations are: A and A′ are voltage-sensing electrodes; a are the air lines to aerate the frog skin; B and B′ are current-passing electrodes; C are the two half-chambers; S is where the skin is inserted between the half-chambers; D is the battery and W the potential divider that are used to adjust (by using current) the voltage across the skin to be equal to zero (i.e., short-circuited) as measured by P the potentiometer (voltmeter); any current-passing the skin while the skin is short-circuited is measured by M the microammeter and is the short-circuited current (μA), thus, active transport. The reproduction of Figure 1 from Ussing and Zerahn (1951) is with kind permission from John Wiley and Sons.
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Comment on

  • A commentary on Active transport of sodium as the source of electric current in the short-circuited isolated frog skin by Ussing, H. H., and Zerahn, R. (1951). Acta Physiol. Scand. 23, 110–127.

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References

    1. Canessa C. M., Schild L., Buell G., Thorens B., Gautschi I., Horisberger J. D., Rossier B. C. (1994). Amiloride-sensitive epithelial Na+ channel is made of three homologous subunits. Nature, 367, 463–46710.1038/367463a0 - DOI - PubMed
    1. Canessa C. M., Horisberger J. D., Rossier B. C. (1993). Epithelial sodium channel related to proteins involved in neurodegeneration. Nature 361, 467–47010.1038/361467a0 - DOI - PubMed
    1. Clarke L. L. (2009). A guide to Ussing chamber studies of mouse intestine. Am. J. Physiol. Gastrointest. Liver Physiol. 296, G1151–G1166 - PMC - PubMed
    1. Hamilton K. L., Eaton D. C. (1985). Single recordings from amiloride-sensitive sodium channels. Am. J. Physiol. 249, C200–C207 - PubMed
    1. Hamilton K. L., Eaton D. C. (1986). Regulation of single sodium channels in renal tissue: a role in sodium homeostasis. Fed. Proc. 45, 2713–2717 - PubMed

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