Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1990 Jun;58(6):1705-10.
doi: 10.1128/iai.58.6.1705-1710.1990.

Identification of Pneumocystis carinii chromosomes and mapping of five genes

B Lundgren  1 , R Cotton, J D Lundgren, J C Edman, J A Kovacs
Affiliations

Identification of Pneumocystis carinii chromosomes and mapping of five genes

B Lundgren et al. Infect Immun. 1990 Jun.

Abstract

Pulsed field gel electrophoresis was used to identify the chromosome-size DNA of Pneumocystis carinii, a major pathogen of immunocompromised patients. Thirteen chromosomes of rodent Pneumocystis carinii, ranging in size from 300 to 700 kilobases (kb), were identified. The minimum genome size for P. carinii, estimated on the basis of the sizes of chromosomes, is 7,000 kb. Genetic heterogeneity among different P. carinii isolates was documented by demonstration of chromosomal size variability. By hybridization studies, the genes for topoisomerase I, dihydrofolate reductase, rRNA, actin, and thymidylate synthase were mapped to single chromosomes of approximately 650, 590, 550, 460, and 350 kb, respectively. Hybridization studies further confirmed the genetic heterogeneity of P. carinii.

PubMed Disclaimer

References

    1. J Mol Biol. 1975 Nov 5;98(3):503-17 - PubMed
    1. Mol Microbiol. 1989 Nov;3(11):1473-80 - PubMed
    1. Proc Natl Acad Sci U S A. 1980 Jul;77(7):3912-6 - PubMed
    1. Anal Biochem. 1983 Jul 1;132(1):6-13 - PubMed
    1. Ann Intern Med. 1984 May;100(5):663-71 - PubMed

Publication types

MeSH terms

LinkOut - more resources