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. 1990 Jun;10(6):3268-71.
doi: 10.1128/mcb.10.6.3268-3271.1990.

Genes transferred by retroviral vectors into normal and mutant myoblasts in primary cultures are expressed in myotubes

B F Smith  1 , R K Hoffman, U Giger, J H Wolfe
Affiliations

Genes transferred by retroviral vectors into normal and mutant myoblasts in primary cultures are expressed in myotubes

B F Smith et al. Mol Cell Biol. 1990 Jun.

Abstract

Retroviral vectors were used to transfer genes efficiently into rat and dog myoblasts in primary cultures under conditions which permitted the transduced myoblasts to differentiate into myotubes expressing the transferred genes. The transduced myotubes expressed normal markers of differentiation and were morphologically indistinguishable from uninfected myotubes. Retroviral vector-mediated gene transfer was also used to correct a genetic enzyme deficiency in mutant canine muscle cells.

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References

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