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. 2011 Jul 7;9(13):4752-5.
doi: 10.1039/c1ob05289f. Epub 2011 May 23.

Photochemically amplified detection of molecular recognition events: an ultra-sensitive fluorescence turn-off binding assay

Tiffany P Gustafson  1 , Greg A Metzel, Andrei G Kutateladze
Affiliations

Photochemically amplified detection of molecular recognition events: an ultra-sensitive fluorescence turn-off binding assay

Tiffany P Gustafson et al. Org Biomol Chem. .

Abstract

Amplified fluorescence quenching methodology based on massive autocatalytic photo-unmasking of a dual function sensitizer-quencher is developed and adopted for photoassisted ultra-sensitive detection of molecular recognition events. The resulting binding assay, based on a molecular recognition-triggered photo-amplified cascade with concomitant decrease of fluorescence is validated with the biotin-avidin pair, achieving attomolar detection.

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