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. 2011 May 26:10:146.
doi: 10.1186/1475-2875-10-146.

Evaluation of allelic forms of the erythrocyte binding antigen 175 (EBA-175) in Plasmodium falciparum field isolates from Brazilian endemic area

Daiana S Perce-da-Silva  1 , Dalma M Banic, Josué C Lima-Junior, Fátima Santos, Cláudio T Daniel-Ribeiro, Joseli de Oliveira-Ferreira, Lilian R Pratt-Riccio
Affiliations

Evaluation of allelic forms of the erythrocyte binding antigen 175 (EBA-175) in Plasmodium falciparum field isolates from Brazilian endemic area

Daiana S Perce-da-Silva et al. Malar J. .

Abstract

Background: The Plasmodium falciparum Erythrocyte Binding Antigen-175 (EBA-175) is an antigen considered to be one of the leading malaria vaccine candidates. EBA-175 mediates sialic acid-dependent binding to glycophorin A on the erythrocytes playing a crucial role during invasion of the P. falciparum in the host cell. Dimorphic allele segments, termed C-fragment and F-fragment, have been found in high endemicity malaria areas and associations between the dimorphism and severe malaria have been described. In this study, the genetic dimorphism of EBA-175 was evaluated in P. falciparum field isolates from Brazilian malaria endemic area.

Methods: The study was carried out in rural villages situated near Porto Velho, Rondonia State in the Brazilian Amazon in three time points between 1993 and 2008. The allelic dimorphism of the EBA-175 was analysed by Nested PCR.

Results: The classical allelic dimorphism of the EBA-175 was identified in the studied area. Overall, C-fragment was amplified in a higher frequency than F-fragment. The same was observed in the three time points where C-fragment was observed in a higher frequency than F-fragment. Single infections (one fragment amplified) were more frequent than mixed infection (two fragments amplified).

Conclusions: These findings confirm the dimorphism of EBA175, since only the two types of fragments were amplified, C-fragment and F-fragment. Also, the results show the remarkable predominance of CAMP allele in the studied area. The comparative analysis in three time points indicates that the allelic dimorphism of the EBA-175 is stable over time.

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Figures

Figure 1
Figure 1
Map of Brazil and Rondonia state.
Figure 2
Figure 2
Nested polymerase chain reaction of EBA-175 products. Lane 1: 200 bp molecular ladder; Lane 2: negative control; Lane 3: PCR positive control (C-fragment); Lane 4: C-fragment of 714 bp; Lane 5: F-fragment of 795 bp; Lane 6: Mixed infection (F- and C-fragment); Lane 7: 200 bp molecular ladder.
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