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. 2011:705:453-64.
doi: 10.1007/978-1-4419-7877-6_24.

Importance of a factor VIIIc-like glycoprotein expressed in capillary endothelial cells (eFactor VIIIc) in angiogenesis

Dipak K Banerjee  1 , Caroline M Oliveira, José J Tavárez, Viswa N Katiyar, Subiman Saha, Juan A Martínez, Aditi Banerjee, Aurymar Sánchez, Krishna Baksi
Affiliations

Importance of a factor VIIIc-like glycoprotein expressed in capillary endothelial cells (eFactor VIIIc) in angiogenesis

Dipak K Banerjee et al. Adv Exp Med Biol. 2011.
No abstract available

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Figures

Fig. 24.1
Fig. 24.1
Domain structure of factor VIII. Arg 372, Arg 740, and Arg 1648 are proteolytic cleavage sites
Fig. 24.2
Fig. 24.2
Expression of GRP-78 in capillary endothelial cells as a function of time. Cells were labeled with [35S]methionine, and GRP-78 was immunoprecipitated from the cell lysate with anti-GRP-78 monoclonal antibody and analyzed by 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) followed by autoradiography
Fig. 24.3
Fig. 24.3
Expression of factor VIIIc in capillary endothelial cells. (a) Cellular localization of factor VIIIc by immunofluorescence microscopy; (b) autoradiography of actively synthesized factor VIIIc; and (c) bioactivity of immunoprecipiated factor VIIIc: 1 = cell lysate; 2 = conditioned media; 3 = bovine plasma; 4 = human plasma; 5 = EMEM with 10% fetal bovine serum; 6 = cell lysate with a protease inhibitor (aprotinine)
Fig. 24.4
Fig. 24.4
Factor VIIIc is an asparagine-linked glycoprotein. Cells were labeled with [35S]methionine, and immunoprecipitated factor VIIIc was digested with N- and O-glycanases and analyzed by SDS-PAGE followed by autoradiography. Lane 1 = control; lane 2 = after digestion with N-glycanase; lane 3 = after digestion with O-glycanase
Fig. 24.5
Fig. 24.5
Effect of isoproterenol on factor VIIIc biosynthesis. The cells were labeled with [35S]methionine for 1 h at 37°C in the presence of isoproterenol, and factor VIIIc was analyzed in the cell lysate and in the conditioned media. Con control; a = 1 × 10−3 M isoproterenol; b = 1 × 10−5 M isoproterenol; c = 1 × 10−7 M isoprotrenol; d = 1 × 10−9 M isoproterenol
Fig. 24.6
Fig. 24.6
Effect of 8Br-cAMP on capillary endothelial cell proliferation and the lumen formation. A synchronized cell population was treated with 2 mM 8Br-cAMP for 0–96 h. The cell numbers were counted in a hemocytometer, and the morphology was monitored under a Nikon PMS microscope. (a) The growth curve; (b) control cells; and (c) cells treated with 2 mM 8Br-cAMP
Fig. 24.7
Fig. 24.7
Downregulation of factor VIIIc expression and cellular proliferation by tunicamycin. Cells were cultured in the absence or in the presence of tunicamycin (1 µg/mL). Factor VIIIc expression was examined after 32 h, whereas the cell number was monitored after every 24 h for 96 h
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References

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