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. 1990 Mar;81(3):272-8.
doi: 10.1111/j.1349-7006.1990.tb02561.x.

Detection of Epstein-Barr virus DNA in Reed-Sternberg cells of Hodgkin's disease using the polymerase chain reaction and in situ hybridization

H Uhara  1 , Y Sato, K Mukai, I Akao, Y Matsuno, S Furuya, T Hoshikawa, Y Shimosato, T Saida
Affiliations

Detection of Epstein-Barr virus DNA in Reed-Sternberg cells of Hodgkin's disease using the polymerase chain reaction and in situ hybridization

H Uhara et al. Jpn J Cancer Res. 1990 Mar.

Abstract

Thirty-one cases of Hodgkin's disease were examined for the occurrence of Epstein-Barr virus (EBV) genome by using the polymerase chain reaction (PCR) of DNA in formalin-fixed paraffin-embedded tissues and in situ hybridization technique. The cases were subdivided into 17 cases of nodular sclerosis (NS), nine cases of mixed cellularity (MC), four cases of lymphocyte predominance (LP), and one case of lymphocyte depletion (LD). EBV DNA was detected in eight cases including four cases of NS, three cases of MC and one case of LP. The sensitivity of PCR was higher than that of Southern blot hybridization of DNA from fresh frozen tissue, because Southern blot hybridization using the BamHI-W fragment of EBV detected virus DNA only in two of three cases which were positive by PCR. The results of in situ hybridization studies confirmed that EBV genome was localized within the nuclei of Reed-Sternberg (RS) cells and their mononuclear variants. Furthermore, double-labeling studies combining in situ hybridization and immunocytochemistry using CD30 (BerH2) and CD15 (LeuM1) as markers of RS cells, as well as pan B-marker (L26) and pan T-marker, CD45RO (UCHL1), were performed to demonstrate the phenotype of EBV DNA-positive cells, confirming that EBV DNA was present in RS cells but not in lymphocytes. The results of this study indicate a significant association between EBV and some cases of Hodgkin's disease.

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References

    1. ) Stein , H. , Mason , D. Y. , Gerdes , J. , O'Connor , N. , Wainscoat , J. , Pallesen , G. , Gatter , K. , Falini , B. , Delsol , G. , Lemke , H. , Schwalting , R. and Lennert , K.The expression of the Hodgkin's disease associated antigen Ki‐1 in reactive and neoplastic lymphoid tissue: evidence that Reed‐Sternberg cells and histiocytic malignancies are derived from activated lymphoid cells . Blood , 66 , 848 – 858 ( 1985. ). - PubMed
    1. ) Kadin , M. E. , Muramato , L. and Said , J.Expression of T‐cell antigens on Reed‐Sternberg cells in a subset of patients with nodular sclerosing and mixed cellularity Hodgkin's disease . Am. J. Pathol. , 130 , 345 – 353 ( 1988. ). - PMC - PubMed
    1. ) Agnarsson , B. A. and Kadin , M. E.The immuno‐phenotype of Reed‐Sternberg cells. A study of 50 cases of Hodgkin's disease using fixed frozen tissues . Cancer , 63 , 2083 – 2087 ( 1989. ). - PubMed
    1. ) Weiss , L. M. , Strickler , J. G. , Hu , E. , Warnke , R. A. and Sklar , J.Immunoglobulin gene rearrangement in Hodgkin's disease . Hum. Pathol. , 17 , 1009 – 1014 ( 1986. ). - PubMed
    1. ) Griesser , H. , Feller , A. , Lennert , K. , Minden , M. and Mak , T. W.Rearrangement of the β chain of the T cell antigen recepter and immunoglobulin genes in lympho‐proliferative disorders . J. Clin. Invest. , 78 , 1179 – 1184 ( 1986. ). - PMC - PubMed

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