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. 2011 Jul 4;50(28):6292-5.
doi: 10.1002/anie.201100202. Epub 2011 May 30.

Electrostatic control of bioactivity

Joshua E Goldberger  1 , Eric J Berns, Ronit Bitton, Christina J Newcomb, Samuel I Stupp
Affiliations

Electrostatic control of bioactivity

Joshua E Goldberger et al. Angew Chem Int Ed Engl. .
No abstract available

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Figures

Figure 1
Figure 1
(a–c, d inset) CryoTEM images of 2–5, in an 150 mM NaCl, 3 mM KCl isotonic salt solution. (d) CryoTEM of 5 dissolved in a 150 mM NaCl, 3 mM KCl, 5 mM CaCl2 salt solution. (e–h, f inset) TEM images of 2–5 from solutions prepared in H2O. In both (b) and (f), black and white arrows refer to trimer bundle motifs, and cylindrical assemblies, respectively. In both (c) and (g), black and white arrows refer to triple helices, and dimer bundle motifs, respectively.
Figure 2
Figure 2
(a) SAXS spectra of synthesized PA molecules at 1 wt% dissolved in 150 mM NaCl, 3 mM KCl, and when noted, 5 mM CaCl2. Curves were shifted vertically for visually convenience. (Inset) Fit of 5 to a spherical micelle model. (b) CD curves of 1 wt% PA solutions dissolved in the same salt solutions.
Figure 3
Figure 3
Representative flattened-stack confocal fluorescence images of neurons cultured in peptide amphiphile gels containing 0.8 wt% 1 with (a) 0.2 wt% 2 and (b) 0.2 wt% 5. Green = β-III-tubulin, Blue = Hoechst. Scale bar = 20 µm. C) Average neurite lengths of neurons cultured in peptide amphiphile gels. n > 59. * p < 0.01 to 1, ^ p < 0.05 to 3.
Scheme 1
Scheme 1
Peptide amphiphile design includes a hydrophobic group (black), β-sheet forming sequence (red), charged amino acids (blue), and a bioactive epitope (green). 1–5 is a list of synthesized peptide amphiphiles.
See this image and copyright information in PMC

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