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. 2011 Aug;49(8):2980-4.
doi: 10.1128/JCM.00431-11. Epub 2011 Jun 1.

Utility of matrix-assisted laser desorption ionization-time of flight mass spectrometry following introduction for routine laboratory bacterial identification

Stephen A Neville  1 , Annabelle Lecordier, Helen Ziochos, Mathew J Chater, Iain B Gosbell, Michael W Maley, Sebastiaan J van Hal
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Utility of matrix-assisted laser desorption ionization-time of flight mass spectrometry following introduction for routine laboratory bacterial identification

Stephen A Neville et al. J Clin Microbiol. 2011 Aug.

Abstract

Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) was evaluated prospectively in a diagnostic laboratory. Nine hundred twenty-seven organisms were tested in triplicate; 2,351/2,781 (85%) species and 2,681/2,781 (96%) genus identifications were correct. Known issues such as the misidentification of alpha-hemolytic streptococci as Streptococcus pneumoniae were easily corrected. Identifications cost AUD$0.45 per isolate and were available in minutes. MALDI-TOF MS is rapid, accurate, and inexpensive.

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References

    1. Anhalt J. P., Fenselau C. 1975. Identification of bacteria using mass spectrometry. Ann. Chem. 47:219–225
    1. Barenfanger J., Drake C., Kacich G. 1999. Clinical and financial benefits of rapid bacterial identification and antimicrobial susceptibility testing. J. Clin. Microbiol. 37:1415–1418 - PMC - PubMed
    1. Christner M., et al. 2010. Rapid identification of bacteria from positive blood culture bottles by use of matrix-assisted laser desorption ionization—time of flight mass spectrometry fingerprinting. J. Clin. Microbiol. 48:1584–1591 - PMC - PubMed
    1. Doern G. V., Vautour R., Gaudet M., Levy B. 1994. Clinical impact of rapid in vitro susceptibility testing and bacterial identification. J. Clin. Microbiol. 32:1757–1762 - PMC - PubMed
    1. Eigner U., et al. 2009. Performance of a matrix-assisted laser desorption ionization-time-of-flight mass spectrometry system for the identification of bacterial isolates in the clinical routine laboratory. Clin. Lab. 55:289–296 - PubMed

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