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. 1990 Jun 1;268(2):267-74.
doi: 10.1042/bj2680267.

Disulphide bond assignment in human tissue inhibitor of metalloproteinases (TIMP)

R A Williamson  1 , F A Marston, S Angal, P Koklitis, M Panico, H R Morris, A F Carne, B J Smith, T J Harris, R B Freedman
Affiliations

Disulphide bond assignment in human tissue inhibitor of metalloproteinases (TIMP)

R A Williamson et al. Biochem J. .

Abstract

Disulphide bonds in human recombinant tissue inhibitor of metalloproteinases (TIMP) were assigned by resolving proteolytic digests of TIMP on reverse-phase h.p.l.c. and sequencing those peaks judged to contain disulphide bonds by virtue of a change in retention time on reduction. This procedure allowed the direct assignment of Cys-145-Cys-166 and the isolation of two other peptides containing two disulphide bonds each. Further peptide cleavage in conjunction with fast-atom-bombardment m.s. analysis permitted the assignments Cys-1-Cys-70, Cys-3-Cys-99, Cys-13-Cys-124 and Cys-127-Cys-174 from these peptides. The sixth bond Cys-132-Cys-137 was assigned by inference, as the native protein has no detectable free thiol groups.

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