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. 2011 Jan;34(1):84-7.
doi: 10.1590/S1415-47572010005000116. Epub 2011 Mar 1.

The karyotype of Adenomera diptyx (Boettger 1885) (Anura, Leptodactylidae) from northeastern Argentina

Affiliations

The karyotype of Adenomera diptyx (Boettger 1885) (Anura, Leptodactylidae) from northeastern Argentina

Víctor Hugo Zaracho et al. Genet Mol Biol. 2011 Jan.

Abstract

In this work we analyzed the karyotype of five populations of Adenomera diptyx from Argentina after conventional staining, Ag-NOR and C-banding. All specimens presented 2n = 26 and FN = 34. The karyotype was formed by three submetacentric, one metacentric and nine telocentric pairs. Silver staining revealed that the NOR was located on a secondary constriction in pair 7. C- banding evidenced constitutive heterochromatin at the pericentromeric region of all chromosomes. The karyotype of A. diptyx was similar to that of A. hylaedactyla (2n = 26, FN = 34) and different from that of A. andreae (2n = 26, FN = 40) in the fundamental number and secondary constriction position. It also differed from the karyotypes of A. marmorata (2n = 24, FN = 34 and 36) and of A. aff. bokermanni (2n = 23, FN = 34) in diploid number. Until a comprehensive cytogenetic analysis of all the species of the genus is performed, their chromosome evolution will remain poorly understood.

Keywords: Adenomera diptyx; Ag-NOR; Argentina; C-bands; standard karyotype.

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Figures

Figure 1
Figure 1
Karyotype of Adenomera diptyx (2n = 26, FN = 34) from northeastern Argentina after (a) Conventional Giemsa staining, note the presence of secondary constrictions on pair 7. Inset: NOR-bearing chromosome pair after silver staining; (b) metaphase after C-banding.

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