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. 2011 Oct 1;87(1):146-50.
doi: 10.1016/j.colsurfb.2011.05.013. Epub 2011 May 12.

Characterisation of exosomes derived from human cells by nanoparticle tracking analysis and scanning electron microscopy

Viktoriya Sokolova  1 , Anna-Kristin Ludwig, Sandra Hornung, Olga Rotan, Peter A Horn, Matthias Epple, Bernd Giebel
Affiliations

Characterisation of exosomes derived from human cells by nanoparticle tracking analysis and scanning electron microscopy

Viktoriya Sokolova et al. Colloids Surf B Biointerfaces. .

Abstract

Exosomes from three different cell types (HEK 293T, ECFC, MSC) were characterised by scanning electron microscopy (SEM), dynamic light scattering (DLS) and nanoparticle tracking analysis (NTA). The diameter was around 110 nm for the three cell types. The stability of exosomes was examined during storage at -20°C, 4°C, and 37°C. The size of the exosomes decreased at 4°C and 37°C, indicating a structural change or degradation. Multiple freezing to -20°C and thawing did not affect the exosome size. Multiple ultracentrifugation also did not change the exosome size.

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