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. 2011 Jun 5;8(7):565-7.
doi: 10.1038/nmeth.1626.

Single-tube linear DNA amplification (LinDA) for robust ChIP-seq

Pattabhiraman Shankaranarayanan  1 , Marco-Antonio Mendoza-Parra, Mannu Walia, Li Wang, Ning Li, Luisa M Trindade, Hinrich Gronemeyer
Affiliations

Single-tube linear DNA amplification (LinDA) for robust ChIP-seq

Pattabhiraman Shankaranarayanan et al. Nat Methods. .

Abstract

Genome-wide profiling of transcription factors based on massive parallel sequencing of immunoprecipitated chromatin (ChIP-seq) requires nanogram amounts of DNA. Here we describe a high-fidelity, single-tube linear DNA amplification method (LinDA) for ChIP-seq and reChIP-seq with picogram DNA amounts obtained from a few thousand cells. This amplification technology will facilitate global analyses of transcription-factor binding and chromatin with very small cell populations, such as stem or cancer-initiating cells.

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References

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