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. 2011 Jun 5;8(7):568-70.
doi: 10.1038/nmeth.1622.

Near-infrared branding efficiently correlates light and electron microscopy

Derron Bishop  1 , Ivana Nikić, Mary Brinkoetter, Sharmon Knecht, Stephanie Potz, Martin Kerschensteiner, Thomas Misgeld
Affiliations

Near-infrared branding efficiently correlates light and electron microscopy

Derron Bishop et al. Nat Methods. .

Abstract

The correlation of light and electron microscopy of complex tissues remains a major challenge. Here we report near-infrared branding (NIRB), which facilitates such correlation by using a pulsed, near-infrared laser to create defined fiducial marks in three dimensions in fixed tissue. As these marks are fluorescent and can be photo-oxidized to generate electron contrast, they can guide re-identification of previously imaged structures as small as dendritic spines by electron microscopy.

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