Detection of infectious bronchitis virus antigen from experimentally infected chickens by indirect immunofluorescent assay with monoclonal antibody
Affiliations
- PMID: 2164384
Item in Clipboard
Detection of infectious bronchitis virus antigen from experimentally infected chickens by indirect immunofluorescent assay with monoclonal antibody
Avian Dis.
1990 Apr-Jun.
Abstract
Infectious bronchitis virus (IBV) was detected by indirect immunofluorescent assay with a monoclonal antibody (MAb-IFA). The monoclonal antibody was specific for the nucleocapsid protein of IBV strain M41. The MAb-IFA clearly detected IBV with high specificity in infected chicken kidney cells. The assay furthermore detected IBV in tracheal smears and sliced tracheas from experimentally infected chickens. The positive reaction was found to be longer than that in the virus recovery test. These results indicate that MAb-IFA is a useful method for the detection of IBV from chickens suspected to have infectious bronchitis.
Similar articles
-
A monoclonal antibody-based immunoperoxidase procedure for rapid detection of infectious bronchitis virus in infected tissues.Avian Dis. 1990 Oct-Dec;34(4):893-8. Avian Dis. 1990. PMID: 2177975
-
Diagnosis of infectious bronchitis (IB) by examination of tracheal mucus for IB-precipitating antigens.Avian Dis. 1981 Oct-Dec;25(4):1058-64. Avian Dis. 1981. PMID: 6279064
-
Detection and quantification of antibodies to infectious bronchitis virus by enzyme-linked immunosorbent assay.Avian Dis. 1981 Jul-Sep;25(3):713-22. Avian Dis. 1981. PMID: 6274297
-
Avian infectious bronchitis vaccine: primordial or derived virus?Dev Biol Stand. 1976;33:311-20. Dev Biol Stand. 1976. PMID: 60263 Review.
-
Avian infectious bronchitis: characteristics of the virus and antigenic types.Am J Vet Res. 1975 Apr;36(4 Pt 2):522-3. Am J Vet Res. 1975. PMID: 47725 Review. No abstract available.
Cited by
-
Pathogenesis and Diagnostic Approaches of Avian Infectious Bronchitis.Adv Virol. 2016;2016:4621659. doi: 10.1155/2016/4621659. Epub 2016 Feb 3. Adv Virol. 2016. PMID: 26955391 Free PMC article. Review.
-
Protein pI alteration related to strain variation of infectious bronchitis virus, an avian Coronavirus.J Virol Methods. 1991 Jun;33(1-2):115-25. doi: 10.1016/0166-0934(91)90012-o. J Virol Methods. 1991. PMID: 1658026 Free PMC article.
-
Pathological assessment and tissue tropism of two different Egyptian infectious bronchitis strains.Virusdisease. 2023 Sep;34(3):410-420. doi: 10.1007/s13337-023-00842-x. Epub 2023 Sep 17. Virusdisease. 2023. PMID: 37780904 Free PMC article.
-
Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in sars patients by enzyme-linked immunosorbent assay.J Clin Microbiol. 2004 Jul;42(7):2884-9. doi: 10.1128/JCM.42.7.2884-2889.2004. J Clin Microbiol. 2004. PMID: 15243033 Free PMC article.
-
Evolution, Interspecies Transmission, and Zoonotic Significance of Animal Coronaviruses.Front Vet Sci. 2021 Oct 18;8:719834. doi: 10.3389/fvets.2021.719834. eCollection 2021. Front Vet Sci. 2021. PMID: 34738021 Free PMC article. Review.