Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2011 Jun 3;5 Suppl 4(Suppl 4):S9.
doi: 10.1186/1753-6561-5-S4-S9.

Toll-Like Receptor 6 differential expression in two pig genetic groups vaccinated against Mycoplasma hyopneumoniae

Katiene Régia Silva Sousa  1 , André Mauric Frossard Ribeiro, Paulo Roberto Nunes Goes, Simone Eliza Facioni Guimarães, Paulo Sávio Lopes, Renata Veroneze, Eliane Gasparino
Affiliations

Toll-Like Receptor 6 differential expression in two pig genetic groups vaccinated against Mycoplasma hyopneumoniae

Katiene Régia Silva Sousa et al. BMC Proc. .

Abstract

Background: Mycoplasma hyopneumoniae is the etiologic agent of enzootic pneumonia, which causes important economic losses to swine industry. The Toll-like receptors (TLRs) are pattern-recognition receptors which detect microbial presence and initiate the innate as well as the adaptative immune defense. Toll-like receptor 6 is a type I transmembrane protein that recognizes bacterial components. The aim of this study was to compare mRNA expression pattern of TLR6 gene in two genetically distinct groups of pigs vaccinated against Mycoplasma hyopneumoniae.

Methods: For each genetic group, peripheral blood was collected just before and 10 days after vaccination from 10 Naturalized Brazilian Piau breed and 10 Commercial White Line serum-negative female piglets. RNA was extracted from peripheral blood mononuclear cells (PBMCs), reverse transcripted and the qRT-PCR performed using SYBR green fluorescence system, using GAPDH gene as endogenous control. Analyses were performed by UNIVARIATE (Shapiro-Wilk test) and MIXED procedures of SAS software (version 9.0).

Results: It was observed significant interaction between breed and vaccination, being the TLR6 mRNA expression higher in the Commercial White line than in the Piau breed after vaccination. Furthermore, there was differential expression before and after vaccination in the Commercial White line.

Conclusions: Analysis of in TLR6 gene expression showed difference between the two distinct genetic groups, however, other TLRs gene expression must be evaluated for a better understanding of innate resistance in the pig concerning Mycoplasma hyopneumoniae infection.

PubMed Disclaimer

References

    1. Akira S, Takeda K. Toll-like receptor signalling. Nat. Rev. Immunol. 2004;4:499–511. doi: 10.1038/nri1391. - DOI - PubMed
    1. Bergman IM, Rosengren JK, Edman K, Edfors I. European wild boars and domestic pigs display different polymorphic patterns in the Toll-like receptor (TLR) 1, TLR2, and TLR6 genes. Immunogenetics. 2010;62:49–58. doi: 10.1007/s00251-009-0409-4. - DOI - PubMed
    1. Guimaraes SEF, Lopes PS. Uso de recursos geneticos nativos no mapeamento genetico de suínos. Ação Ambiental. 2001;15(3):27–28.
    1. Kumar H, Kawai T, Akira S. Toll-like receptors and innate immunity. Biochemical and Biophysical Research Communications. 2009;388:621–625. doi: 10.1016/j.bbrc.2009.08.062. - DOI - PubMed
    1. Lazarus R, Vercelli D, Palmer LJ, Klimecki WJ, Silverman EK, Richter B, Riva A, Ramoni M, Martinez FD, Weiss ST, Kwiatkowski DJ. Single nucleotide polymorphisms in innate immunity genes: abundant variation and potential role in complex human disease. Immunol. Rev. 2002;190:9–25. doi: 10.1034/j.1600-065X.2002.19002.x. - DOI - PubMed