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. 2011 Aug;55(8):3929-31.
doi: 10.1128/AAC.00226-11. Epub 2011 Jun 6.

Emergence of NDM-1 metallo-β-lactamase in Pseudomonas aeruginosa clinical isolates from Serbia

Branko Jovcic  1 , Zorica Lepsanovic, Vesna Suljagic, Gorjana Rackov, Jelena Begovic, Ljubisa Topisirovic, Milan Kojic
Affiliations

Emergence of NDM-1 metallo-β-lactamase in Pseudomonas aeruginosa clinical isolates from Serbia

Branko Jovcic et al. Antimicrob Agents Chemother. 2011 Aug.

Abstract

This work reports, for the first time, the presence of New Delhi metallo-β-lactamase 1 (NDM-1) in Pseudomonas aeruginosa. Moreover, this is the first report of the NDM-1 presence in the Balkan region. Cosmid gene libraries of carbapenem-nonsusceptible Pseudomonas aeruginosa clinical isolates MMA83 and MMA533 were screened for the presence of metallo-β-lactamases. Accordingly, both MMA83 and MMA533 carried the bla(NDM-1) gene. Pulsed-field gel electrophoresis (PFGE) analysis indicated that strains MMA83 and MMA533 belonged to different clonal groups. Five additional isolates from different patients clonally related to either MMA83 or MMA533 were found to be NDM-1 positive.

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Figures

Fig. 1.
Fig. 1.
PFGE profiles of P. aeruginosa isolates carrying the blaNDM-1 gene (MMA18, MMA83, MMA434, MMA533, MMA90, MMA766, and MMA795). L, λ concatemers (New England Biolabs).
See this image and copyright information in PMC

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