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Comparative Study
. 2011 Aug;49(8):3065-7.
doi: 10.1128/JCM.00491-11. Epub 2011 Jun 8.

Comparison of two molecular methods for rapid diagnosis of extrapulmonary tuberculosis

Manuel Causse  1 , Pilar Ruiz, Juan Bautista Gutiérrez-Aroca, Manuel Casal
Affiliations
Comparative Study

Comparison of two molecular methods for rapid diagnosis of extrapulmonary tuberculosis

Manuel Causse et al. J Clin Microbiol. 2011 Aug.

Abstract

Application of real-time PCR for the detection of Mycobacterium tuberculosis enables results to be obtained in about 2 h. A total of 340 nonrespiratory samples were processed using two real-time PCR assay kits: Xpert MTB/RIF and Cobas TaqMan MTB. The sensitivity and specificity of the Xpert assay were 95% and 100%, respectively, compared to 78% and 98% for the Cobas assay.

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References

    1. Alcala L., et al. 2001. Evaluation of the upgraded amplified Mycobacterium tuberculosis direct test (Gen-Probe) for direct detection of Mycobacterium tuberculosis in respiratory and non-respiratory specimens. Diagn. Microbiol. Infect. Dis. 41:51–56 - PubMed
    1. Blakemore R., et al. 2010. Evaluation of the analytical performance of the Xpert MTB/RIF assay. J. Clin. Microbiol. 48:2495–2501 - PMC - PubMed
    1. Boehme C. C., et al. 2010. Rapid molecular detection of tuberculosis and rifampin resistance. N. Engl. J. Med. 363:1005–1015 - PMC - PubMed
    1. Carpentier E., et al. 1995. Diagnosis of tuberculosis by Amplicor Mycobacterium tuberculosis test: a multicenter study. J. Clin. Microbiol. 33:3106–3110 - PMC - PubMed
    1. Centers for Disease Control and Prevention 2009. Updated guidelines for the use of nucleic acid amplification tests in the diagnosis of tuberculosis, p. 7–10 Centers for Disease Control and Prevention, Atlanta, GA - PubMed

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