Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2012 Aug;93(1):18-22.
doi: 10.1016/j.rvsc.2011.05.009. Epub 2011 Jun 11.

Characterization of enterotoxigenic E. coli (ETEC), Shiga-toxin producing E. coli (STEC) and necrotoxigenic E. coli (NTEC) isolated from diarrhoeic Mediterranean water buffalo calves (Bubalus bubalis)

G Borriello  1 , M G Lucibelli, E De Carlo, C Auriemma, D Cozza, G Ascione, F Scognamiglio, G Iovane, G Galiero
Affiliations

Characterization of enterotoxigenic E. coli (ETEC), Shiga-toxin producing E. coli (STEC) and necrotoxigenic E. coli (NTEC) isolated from diarrhoeic Mediterranean water buffalo calves (Bubalus bubalis)

G Borriello et al. Res Vet Sci. 2012 Aug.

Abstract

Two hundred and twenty Escherichia coli isolates from 314 Mediterranean water buffalo calves less than 4 weeks old affected by severe diarrhoea with a lethal outcome were characterized for the presence of the virulence factors LT, ST, Stx1, Stx2, haemolysins, intimin, CNF1, CNF2, CDT-I, CDT-II, CDT-III, CDT-IV, and F17-related fimbriae (F17a, F17b, F17c, F17d). The prevalence of ETEC, STEC and NTEC were 1.8%, 6.8% and 20.9%, respectively. The ETEC isolates were all LT-positive and ST-negative. The STEC isolates were all Stx and intimin-positive, with Stx1 (80%) more frequent than Stx2 (27%). The NTEC isolates were all CNF and Hly-positive, with CNF2 (83%) more frequent than CNF1 (22%). Susceptibility assays to 11 antimicrobials displayed high rates of resistance (>30%) to antimicrobials tested. These data show that the most prevalent strains in diarrhoeic water buffalo calves were NTEC, mostly CNF2 and HlyA-positive, with strong associations CNF2/CDT-III and CNF2/F17c.

PubMed Disclaimer

References

    1. Acres S.D. Enterotoxigenic Escherichia coli infections in newborn calves: a review. Journal of Dairy Science. 1985;68:229–256. - PMC - PubMed
    1. Bertin Y., Martin C., Oswald E., Girardeau J.P. Rapid and specific detection of F17-related pilin and adhesin genes in diarrheic and septicemic Escherichia coli strains by multiplex PCR. Journal of Clinical Microbiology. 1996;34:2921–2928. - PMC - PubMed
    1. Beutin L., Montenegro M.A., Orskov I., Orskov F., Prada J., Zimmermann S., Stephan R. Close association of verotoxin (Shiga-like toxin) production with enterohemolysin production in strains of Escherichia coli. Journal of Clinical Microbiology. 1989;27:2559–2564. - PMC - PubMed
    1. Blanco M., Blanco J., Blanco J.E., Ramos J. Enterotoxigenic, verotoxigenic, and necrotoxigenic Escherichia coli isolated from cattle in Spain. American Journal of Veterinary Research. 1993;54:1446–1451. - PubMed
    1. Burns A.L., Ball H.J., Finlay D.A. CNF producing Escherichia coli isolated from cattle in Northern Ireland. Veterinary Microbiology. 1996;49:235–241. - PubMed

MeSH terms