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. 2011 Sep;55(9):4469-74.
doi: 10.1128/AAC.00126-11. Epub 2011 Jun 13.

Pharmacokinetics/pharmacodynamics of colistin and imipenem on mucoid and nonmucoid Pseudomonas aeruginosa biofilms

Wang Hengzhuang  1 , Hong Wu, Oana Ciofu, Zhijun Song, Niels Høiby
Affiliations

Pharmacokinetics/pharmacodynamics of colistin and imipenem on mucoid and nonmucoid Pseudomonas aeruginosa biofilms

Wang Hengzhuang et al. Antimicrob Agents Chemother. 2011 Sep.

Abstract

The time course of activity of colistin and imipenem against mucoid and nonmucoid Pseudomonas aeruginosa growing in a biofilm showed that compared with those for planktonic bacteria, the kinetics of colistin and imipenem retained the concentration- and time-dependent killing, respectively, but higher doses of antibiotics and longer dosing periods were required for biofilm eradication. Biofilms of mucoid P. aeruginosa were more difficult to eradicate than nonmucoid biofilms.

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Figures

Fig. 1.
Fig. 1.
MBC and MBEC: comparison of susceptibilities of colistin and imipenem on planktonic and day 1, day 3, and day 7 biofilm cells of P. aeruginosa PAO1 (nonmucoid), PDO300 (mucoid), and PAO579 (clinical isolate).
Fig. 2.
Fig. 2.
Kill curves of planktonic Pseudomonas aeruginosa nonmucoid PAO1 and mucoid PDO300 with imipenem and colistin. (a) PAO1, planktonic, imipenem; (b) PDO300, planktonic, imipenem; (c) PAO1, planktonic, colistin; (d) PDO300, planktonic, colistin.
Fig. 3.
Fig. 3.
Kill curves of biofilm-growing Pseudomonas aeruginosa nonmucoid PAO1 and mucoid PDO300 with imipenem and colistin. (a) PAO1, day 1 biofilm, imipenem; (b) PDO300, day 1 biofilm, imipenem; (c) PAO1, day 3 biofilm, imipenem; (d) PDO300, day 3 biofilm, imipenem; (e) PAO1, day 1 biofilm, colistin; (f) PDO300, day 1 biofilm, colistin; (g) PAO1, day 3 biofilm, colistin; (h) PDO300, day 3 biofilm, colistin. OD650, optical density at 650 nm.
Fig. 4.
Fig. 4.
Pharmacokinetics in mouse serum of colistin and imipenem versus MIC, MBC, MBIC, and MBEC of P. aeruginosa PAO165307. ■, 16 mg/kg of colistin; ●, 64 mg/kg of imipenem with one-dose intraperitoneal administration.
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References

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