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. 1990 Jun;31(2):165-72.
doi: 10.1002/jmv.1890310216.

Characterization of a nucleic acid probe for the diagnosis of human coronavirus 229E infections

S Myint  1 , D Harmsen, T Raabe, S G Siddell
Affiliations

Characterization of a nucleic acid probe for the diagnosis of human coronavirus 229E infections

S Myint et al. J Med Virol. 1990 Jun.

Abstract

A cDNA copy of the HCV229E nucleocapsid protein gene was isolated and characterized. Sequence analysis predicts a nucleocapsid polypeptide of 389 amino acids with a molecular weight (mol. wt.) of 43,450. Single strand RNA probes derived from the cDNA copy hybridize specifically to HCV229E RNA and approximately 50 pg of intracellular viral RNA can be readily detected. The application of nucleic acid hybridization as a routine procedure for the diagnosis of HCV229E infection is discussed.

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References

    1. Al‐Nakib W, Stanway G, Forsyth M, Hughes PJ, Almond JW, Tyrrell DAJ (1986): Detection of human rhinoviruses and their molecular relationship using cDNA probes. Journal of Medical Virology 20: 289–296. - PMC - PubMed
    1. Baric RS, Stohlman SA, Razavi MK, Lai MMC (1985): Characterization of leader‐related small RNAs in coronavirus‐infected cells: Further evidence for leader‐primed mechanism of transcription. Virus Research 3: 19–33. - PMC - PubMed
    1. Contreras R, Cheroutre H, Degrave W, Fiers W (1982): Simple efficient in vitro synthesis of capped RNA useful for direct expression of cloned DNA. Nucleic Acids Research 10: 6353–6362. - PMC - PubMed
    1. Devereux J, Haeberli P, Smithies O (1984): A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Research 12: 387–395. - PMC - PubMed
    1. Gubler U, Hoffman BJ (1983): A simple and very efficient method for generating cDNA libraries. GENE 25: 263–269. - PubMed

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