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. 1990 Jul;56(7):2251-4.
doi: 10.1128/aem.56.7.2251-2254.1990.

Cloning of alpha- and beta-galactosidase genes from an extreme thermophile, Thermus strain T2, and their expression in Thermus thermophilus HB27

Y Koyama  1 , S Okamoto, K Furukawa
Affiliations

Cloning of alpha- and beta-galactosidase genes from an extreme thermophile, Thermus strain T2, and their expression in Thermus thermophilus HB27

Y Koyama et al. Appl Environ Microbiol. 1990 Jul.

Abstract

The genes encoding thermostable alpha- and beta-galactosidases from an extremely thermophilic bacterium, Thermus strain T2, were cloned in Escherichia coli. The alpha-galactosidase gene was located just downstream from the beta-galactosidase gene. The genes were introduced into Thermus thermophilus HB27 with the aid of Thermus cryptic plasmid pTT8, and beta-galactosidases were expressed constitutively.

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