The fatty acid constitution and ordering state of membranes in dominant temperature-sensitive lethal mutation and wild-type Drosophila melanogaster larvae

Abstract

The ordering state and changes in fatty acid composition of microsomal (MS) and mitochondrial (MC) membranes of two dominant temperature-sensitive (DTS) lethal mutations and the wild-type Oregon-R strain larvae of Drosophila melanogaster have been studied at 18 and 29 degrees C and after temperature-shift experiments. The membranes of wild-type larvae have a stable ordering state, with "S" values between 0.6 (18 degrees C) and 0.5 (29 degrees C) in both membranes which remained unchanged in shift experiments, although the ratios of saturated/unsaturated fatty acids were changed as expected. The strongly DTS mutation 1(2) 10DTS forms very rigid membranes at the restrictive temperature (29 degrees C) which cannot be normalized after shift down, while shift up or development at the permissive temperature results in normal ordering state. This mutant is less able to adjust MS and MC fatty acid composition in response to the growth temperature than the wild type. The less temperature-sensitive 1(2)2DTS allele occupies an intermediate state between Oregon-R and 1(2)10DTS in both respects. We assume and the genetical data suggest that the DTS mutant gene product is in competition with the wild-type product, resulting in a membrane structure which is not able to accommodate to the restrictive temperature.