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. 1990 Sep;172(9):5511-3.
doi: 10.1128/jb.172.9.5511-5513.1990.

Efficient incorporation of galactose into lipopolysaccharide by Escherichia coli K-12 strains with polar galE mutations

C A Schnaitman  1 , E A Austin
Affiliations

Efficient incorporation of galactose into lipopolysaccharide by Escherichia coli K-12 strains with polar galE mutations

C A Schnaitman et al. J Bacteriol. 1990 Sep.

Abstract

Efficient incorporation of exogenous galactose into lipopolysaccharide was observed in a strain with a galE::Tn10 insertion and a strain with a deletion of galOPE. No incorporation was observed in a strain with a longer (galETK) deletion, indicating that incorporation into the galE mutants was due to a low level of expression of galTK from internal or fortuitous promoters. These galE mutants provided a convenient and specific way to radiolabel lipopolysaccharide.

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