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. 2011 Jul 28;47(28):8115-7.
doi: 10.1039/c1cc12685g. Epub 2011 Jun 20.

Facile fabrication of recyclable and active nanobiocatalyst: purification and immobilization of enzyme in one pot with Ni-NTA functionalized magnetic nanoparticle

Wen Wang  1 , Daniel I C Wang, Zhi Li
Affiliations

Facile fabrication of recyclable and active nanobiocatalyst: purification and immobilization of enzyme in one pot with Ni-NTA functionalized magnetic nanoparticle

Wen Wang et al. Chem Commun (Camb). .

Abstract

Ni-NTA functionalized iron oxide magnetic nanoparticle was synthesized and used to selectively immobilize a his-tagged enzyme from cell free extract as an active and recyclable nanobiocatalyst, where purification and immobilization of the target enzyme were accomplished in one pot.

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