Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1990 Aug;81(8):707-10.
doi: 10.1111/j.1349-7006.1990.tb02631.x.

Isolation of an amplified DNA sequence in stomach cancer

H Nakatani  1 , H Sakamoto, T Yoshida, J Yokota, E Tahara, T Sugimura, M Terada
Affiliations

Isolation of an amplified DNA sequence in stomach cancer

H Nakatani et al. Jpn J Cancer Res. 1990 Aug.

Abstract

By use of the in-gel DNA renaturation method, the presence of amplified DNA sequences was demonstrated in KATO-III, a cell line established from a signet ring cell carcinoma of the stomach. A DNA fragment from one of these amplified regions in KATO-III cells was cloned and designated SAM0.2; the locus containing the SAM0.2 fragment was referred to as SAM. The SAM locus was shown to be amplified not only in KATO-III cells, but also in three of 24 surgical specimens of stomach cancers and in two of 13 xenografts of human stomach cancers, all of these specimens being poorly differentiated adenocarcinoma or mucinous adenocarcinoma of the stomach. The SAM locus was not amplified in 14 cell lines of cancers of other organs or in 42 surgical specimens of lung cancers.

PubMed Disclaimer

References

    1. ) Merkel , D. E. , Dressler , L. G. and McGuire , W. L.Flow cytometry, cellular DNA content, and prognosis in human malignancy . J. Clin. Oncol. , 5 , 1690 – 1703 ( 1987. ). - PubMed
    1. ) Sandberg , A. A. , Turc‐Carel , C. and Gemmill , R. M.Chromosomes in solid tumors and beyond . Cancer Res. , 48 , 1049 – 1059 ( 1988. ). - PubMed
    1. ) Taya , Y. , Terada , M. and Sugimura , T.Role of oncogene amplification in tumor progression . Adv. Viral Oncol. , 7 , 141 – 153 ( 1987. ).
    1. ) Alitalo , K. and Schwab , M.Oncogene amplification in tumor cells . Adv. Cancer Res. , 47 , 235 – 281 ( 1986. ). - PubMed
    1. ) Nakatani , H. , Tahara , E. , Sakamoto , H. , Terada , M. and Sugimura , T.Amplified DNA sequences in cancers . Biochem. Biophys. Res. Commun. , 130 , 508 – 514 ( 1985. ). - PubMed

Publication types

MeSH terms

LinkOut - more resources